Wednesday, October 26, 2011

Is the FISH technology cost effective? (series 5 of 5)

I found this article for our series on metal on metal testing for carcinogens for hip patients (read 4  prior posts to come up to speed.)  This was published in 2008 so I am guessing that the technology has come a long way since then.  This abstract addresses the cost effectiveness of the testing.

Will try to get some calls made in the next few days to see how well this process fits the hip testing for potential carcinogens.


Lab Chip. 2008 Dec;8(12):2151-6. Epub 2008 Oct 23.
An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization.

Source

Department of Electrical and Computer Engineering, 2nd Floor ECERF, University of Alberta, Edmonton, CanadaT6G 2V4.
Abstract

Fluorescence in situ hybridization (FISH) is a powerful technique for probing the genetic content of individual cells at the chromosomal scale. Conventional FISH techniques provide a sensitive diagnostic tool for the detection of chromosomal alterations on a cell-by-cell basis; however, the cost-per-test in terms of reagent and highly qualified labour has prevented its wide-spread utilization in clinical settings. Here, we address the inefficient use of labour with the first integrated and automated on-chip FISH implementation, one that requires only minutes of setup time from the technician. Our microfluidic chip has lowered the reagent use by 20-fold, decreased the labour time by 10-fold, and substantially reduced the amount of support equipment needed. We believe this cost-effective platform will make sensitive FISH techniques more accessible for routine clinical usage.
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Microfluidic chip that lowered the cost-per-test of FISH by 90%.


Recently, the first demonstration of Metaphase FISH on chip has led to renewed efforts towards automating the metaphase FISH protocol.[10] Metaphase FISH had continued to be difficult to integrate owing to the complex sample preparation protocol often spanning over 3 weeks. New reports confirm that a research group in Denmark have tested successfully a novel lab on chip device to integrate the entire sample preparation protocol for Metaphase FISH called FISHprep.


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